Cy5 secondary antibody

Author: ohhy

August undefined, 2024

WebAPC secondary antibodies for flow cytometry Comparison of the brightness of Alexa Fluor 647 and Cy5 dye antibody conjugates (prepared in our laboratories). More Alexa Fluor 647 dye molecules can be attached to proteins and nucleic acids without significant quenching, thus yielding conjugates that are much brighter than those possible using the ... WebIL-18 antibody (ABIN5565709). Validated for EIA, RIA. Tested in Human. Order online.

Cy5streptavidin Secondary Antibody Bioz Ratings For Life …

WebAnti-Cy5 antibody [CY5-15] Description Mouse monoclonal [CY5-15] to Cy5 Host species Mouse Tested applications Suitable for: Dot blot, ELISA, IP, ICC, IHC-FoFr, IHC-FrFl, Flow Cyt more details Species reactivity Reacts with: Species independent Immunogen Full length protein corresponding to Cy5. A mixture of proteins labelled with Cy5. WebCan replace standard fluorophores for most applications ( Table 3) Produce the brightest and most photostable conjugates, for multiplexing and multicolor detection See all Alexa Fluor Primary Antibodies See all … dky native plant society

FRET–CLSM and double-labeling indirect immunofluorescence to …

Webab6908 was used as secondary antibody to visualize various rabbit anti-PAK primary antibodies (red). (From Figure 2 of Hennenberg et al) Immunohistochemistry … WebJul 28, 2024 · 4) Mix 0.5 M KPBS-T with 0.1% Triton + 2% Normal Donkey Serum + Secondary Antibody (I have tried 1:100 and 1:200 dilutions for secondary) Incubate 1hr at room temp in dark. 5) Rinse 0.5 M KPBS 3 x ... WebJul 31, 2024 · The cells were treated with either 0.5 μM antibody alone or 0.5 μM Cy5-labeled antibody-MORF1 for 1 h at 37 °C followed by PBS wash and 0.5 μM HSA- ... DARA crosslinked with a secondary antibody (goat antihuman: GAH), and a positive control treatment with CCCP. Depolarization was measured by JC-1 red/green fluorescence by … dkzee cleaning

Anti-Cy3 / Cy5 antibody [CY96] (ab52060) Abcam

Secondary Antibodies - Jackson ImmunoResearch

WebWe offer a broad range of Alexa Fluor 555 products including protein and antibody labeling kits, secondary antibodies, bioconjugates, and more: Super-resolution microscopy (SRM) applications; Antibody and protein labeling kits ; Streptavidin conjugates for signal amplification; Tyramide labeling kits for ultimate sensitivity http://www.drmr.com/abcon/Cy5.html dky thi toeicWebJul 7, 2024 · Since Cy5 does not survive gelation 1,3,5, we performed an additional experiment labeling the oligonucleotide-modified secondary antibodies after expansion with complementary Cy5-modified ... crazy bouncing

"WebThis Cy5-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Cy5 … " - Cy5 secondary antibody

Cy5 secondary antibody

WebAug 13, 2024 · Santa Cruz secondary antibodies are commonly affinity purified against immobilized whole IgG isotypes, including IgG 1, IgG 2a, IgG 2b, IgG 3 and IgG 4. … WebCyanine Cy™5. Amax: 650 Emax: 670nm. Cy5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm. They can be excited to about 98% of maximum with …

Did you know?

WebGeneral notes. Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5. The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant ... WebMouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A10524) in ICC/IF. Immunofluorescence analysis of A-11064, A-11077 and A10524 was performed using primary antibodies against Pericentrin (Product # PA5-53498), alpha Tubulin (Product # MA1-80017) and ATP5A1 (Product # 43-9800) in 70% confluent log phase HEK 293 cells.

WebRat IgG (H+L) Cross-Adsorbed Secondary Antibody (A10525) in ICC/IF Immunofluorescence analysis of Goat anti-Rat IgG (H+L) Secondary Antibody, Cy5® was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). WebThis PerCP-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar PerCP goat anti-human IgG antibodies from other commercial sources, and thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility ...

WebSecondary antibodies ELISA and Matched Antibody Pair Kits Cell and tissue imaging tools Cellular and biochemical assays Proteins and Peptides By product type ... WebOverview: Catalog # bs-9135R-Cy5: Product Name: ASB17 Polyclonal Antibody, Cy5 Conjugated: Applications: WB, IF(IHC-P) Reactivity: Human, Mouse, Rat: Specifications

WebApr 18, 2008 · Cy5® Conjugation Kit (Fast) - Lightning-Link® (ab188288) Blocking . Normal Donkey Serum (ab7475) Secondary ... Looking for unlabeled secondary antibodies, as well as isotype control antibodies in preparations lacking BSA. I'm not sure what "H&L" means, so I'm holding off on ordering ab6701. Thanks,

WebDilute the fluorophore-conjugated secondary antibody in PBS-0.05% Tween 20 (again you can include 5% goat serum if the secondary antibodies are from goat). Most … crazy bouncing ballWebKey features and details Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm Host species: Goat Isotype: IgG Suitable for: Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Fr Related conjugates and formulations dky shopee foodWebSpecializing in Secondary Antibodies and Conjugates - For Western Blotting, IHC, ICC, Flow Cytometry, ELISA and other immunological applications. 800-367-5296; … crazy bout a mercury