Tīmeklis2013. gada 1. jūn. · The samples were added to EzApply (ATTO, Tokyo, Japan), adjusted to a final protein concentration of 1 µg/μl, and then boiled at 95 °C for 5 minutes. We then applied 10 µg protein to a 12.5% polyacrylamide gel (ATTO, Tokyo, Japan). Electrophoresis was carried out at a constant current of 20 mA/gel for 60 … Tīmeklis2024. gada 8. nov. · EzGel Ace and EzRun MOPS (Atto) were used as the buffer for …
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Tīmeklis型式・名称. AE-1435 EzApply 2D Kit (イージーアプライ2Dキット) 主成分. Wash … Tīmeklis2024. gada 19. maijs · Human synovial fluids and cell lysate were mixed samples buffer EzApply (ATTO, Osaka, Japan) and heated at 100°C for 5 min. The extract proteins were subjected to SDS-PAGE gels and transferred electrophoretically onto polyvinylidene difluoride membrane (Immobilon-P Membrane; Merck, Darmstadt, … dominick \u0026 daughters
EzRun 電気泳動・泳動バッファー 試薬 アトー製品情報 ATTO …
Tīmeklisbuffer (EzApply; Atto, Tokyo, Japan). The protein sampleswereboiledandstoredat 20 C untiluse.As described previously [12], the proteins were fraction-ated with sodium dodecyl sulfate polyacrylamide gel electrophoresis (10% linear gel; e-PAGEL, Atto) and Figure 1. (continued). g-H2AX and p53 representing cell quality 115 Tīmeklis通过将目的蛋白与具有下述氨基酸序列的肽标签连接并表达,将目的蛋白高效蓄积在可溶性级分中:X(PY)qPZ,P表示脯氨酸,X表示由0~5个氨基酸组成的氨基酸序列,所述氨基酸独立选自由精氨酸(R),甘氨酸(G),丝氨酸(S),赖氨酸(K),苏氨酸(T),亮氨酸(L),天冬酰胺(N),谷氨酰胺(Q)及甲硫氨酸(M)组成的 ... TīmeklisEzApply 2D Kit. WSE-1435. Sample preparation buffer for 2D. EzApply. WSE-1430. … dominick's pizza union blvd totowa nj