WebJan 1, 2008 · The reported assay, which is based on the BacTiter-Glo assay from Promega, uses bioluminescence to detect the intracellular concentration of ATP, which correlates with viable bacterial cell... WebBacTiter-Glo™ Microbial Cell Viability Assay INSTRUCTIONS FOR USE OF PRODUCTS G8230, G8231, G8232 AND G8233. PROTOCOL Quick Before beginning the protocol, see Section 3.A., Reagent Preparation, in the BacTiter-Glo™ Microbial Cell Viability Assay Technical Bulletin , #TB337. 1. Perform the following steps at room temperature …
CellTiter-Glo® Luminescent Cell Viability Assay - Promega
WebThe CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. WebJun 5, 2012 · Promega 成功地开发出基于另一种萤火虫 Photinus pennsylvanica 的一种稳定形式的萤光素酶(Ultra-Glo TM 重组萤光素酶),使用一种能选 择在ATP 检测性能方面有所提高的性质。 另外,我们开发了一个专利配方,可以从不同 的微生物细胞中快速有效地提取ATP(表1)。 BacTiter-Glo TM 试剂中这两个基本因素的 结合使我们能够设计出在培养 … inexpensive queen mattress and boxspring
Protocol for Measuring ATP from Bacteria - Promega
WebBacTiter-Glo™ Microbial Cell Viability Assay a Kit by Promega. J774A.1; Monocyte-macrophage; Mouse (Mus musculus) an Experimental Model by ATCC. DMEM, high glucose a Biochemical by Invitrogen Antibodies. Laurdan a Biochemical by Cayman Chemical ... WebThe CellTiter-Glo® 3D Cell Viability Assay is designed for determining cell viability in 3D microtissue spheroids. The assay reagent penetrates large spheroids and has increased lytic capacity—allowing more accurate determination of viability … WebThe antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay (BacTiter-Glo; Promega, Madison, WI, USA) and confocal laser scanning microscopy (CLSM). ... The luminescence produced was measured with a luminometer (GloMax; Promega, Madison, WI, USA), expressed as absolute values of relative light units ... inexpensive queen size sheet sets